SARS Coronavirus Detection
Identifieur interne : 005154 ( Main/Exploration ); précédent : 005153; suivant : 005155SARS Coronavirus Detection
Auteurs : Andreas Nitsche [Allemagne] ; Brunhilde Schweiger [Allemagne] ; Heinz Ellerbrok [Allemagne] ; Matthias Niedrig [Allemagne] ; Georg Pauli [Allemagne]Source :
- Emerging Infectious Diseases [ 1080-6040 ] ; 2004.
Descripteurs français
- KwdFr :
- MESH :
- analyse : ARN viral.
- génétique : Virus du SRAS.
- isolement et purification : Virus du SRAS.
- virologie : Syndrome respiratoire aigu sévère.
- Humains, RT-PCR, Sensibilité et spécificité.
English descriptors
- KwdEn :
- MESH :
- chemical , analysis : RNA, Viral.
- genetics : SARS Virus.
- isolation & purification : SARS Virus.
- virology : Severe Acute Respiratory Syndrome.
- Humans, Reverse Transcriptase Polymerase Chain Reaction, Sensitivity and Specificity.
Abstract
We developed a set of three real-time reverse transcription–polymerase chain reaction (PCR) assays that amplify three different regions of the SARS-associated coronavirus (SARS-CoV), can be run in parallel or in a single tube, and can detect <10 genome equivalents of SARS-CoV. The assays consider all currently available SARS-CoV sequences and are optimized for two prominent real-time PCR platforms.
Url:
DOI: 10.3201/eid1007.030678
PubMed: 15324554
PubMed Central: 3323308
Affiliations:
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Le document en format XML
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<front><div type="abstract" xml:lang="en"><p>We developed a set of three real-time reverse transcription–polymerase chain reaction (PCR) assays that amplify three different regions of the SARS-associated coronavirus (SARS-CoV), can be run in parallel or in a single tube, and can detect <10 genome equivalents of SARS-CoV. The assays consider all currently available SARS-CoV sequences and are optimized for two prominent real-time PCR platforms.</p>
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